Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Si-Miao-Yong-An on promoting the maturation of Vasa Vasorum and stabilizing atherosclerotic plaque in ApoE -/- mice： An experimental study.

doi: 10.1016/j.biopha.2019.108785

Figure Lengend Snippet: Fig. 3. Effect of Si-Miao-Yong-An (SMYA) on VV maturation in ApoE−/−mice. VV pericytes analysis by immunofluorescence double staining, endothelial cells were labeled with CD34, pericytes were labeled with Desmin. The results of immunofluorescence double staining showed that there was no plaque formation in aorta in the normal group. In the model group, a small pericytes collected new VV in aortic plaques. Compared with the model group, the density of mature VV in plaques in two treatment groups increased significantly (Magnification, ×400).

Article Snippet: Si-Miao-Yong-An (Lyophilized powder was composed of Scrophularia ningpoensis Hemsl., Lonicera japonica., Angelica Sinensis., Glycyrrhiza uralensis Fisch, preparing according to the ratio 3:3:2:1 and purchased from the first affiliated hospital of Tianjin university of Traditional Chinese Medicine); Simvastatin (Hangzhou MSD pharmaceutical co., Ltd., Hangzhou, China, specification was 20mg/tablets, Lot number: H20130146); L-methionine (M5308, sigma, USA); Hematoxylin-eosin staining kit (Tianjin baihao biotechnology co., Ltd.); Fluorescence antibody diluent (A1840, Beijing solaibao technology co., Ltd.); CD34 rat monoclonal antibody (GTX28158, GeneTex, USA); Desmin rabbit monoclonal antibody (ab32362, abcam); streptavidin–biotin complex with peroxidase (SABC-POD, Beijing zhongshan jinqiao biotechnology co., Ltd.) (rabbit IgG) ready-to-use kits and DAB Chromogenic Reagent Kit (Boster Biological Engineering Co., Ltd.); PrimeScriptTM RT reagent Kit (RR037A, Beijing baori medical technology co., Ltd.); SYBR®Premix Ex TaqTM II (RR420A, Beijing baori medical technology co., Ltd.).

Techniques: Double Staining, Labeling

Journal: Blood

Article Title: High endothelial venules as traffic control points maintaining lymphocyte population homeostasis in lymph nodes.

doi: 10.1182/blood-2011-07-367409

Figure Lengend Snippet: Figure 2. HEV pockets are plastic structures. (A) The right panel in the diagram represents a situation in which lymphocytes are not engulfed within pockets but simply migrating between 2 ECs while the left panel represents lymphocytes present in a true structured pocket underneath the HEV. L Lymphocyte. (B) Confocal image of a LN section from a ubiquitin-GFP chimera (green) stained for ICAM-1 (red) and sytox 63/nuclei (blue) Arrowheads indicate the inner membrane of the EC directly in contact with lymphocytes. (C) Confocal image of a LN section from a ubiquitin-GFP chimera (green) stained for ERTR-7 (red), Desmin (white) and sytox 63/nuclei (blue).Arrows indicate the membranes of myofibroblasts/pericytes that ensheath the conduit system. Data are representative of 3 different experiments (2 mice per experiment).

Article Snippet: 6115BLOOD, 1 DECEMBER 2011 VOLUME 118, NUMBER 23 D ow nloaded from http://ashpublications.org/blood/article-pdf/118/23/6115/1344964/zh804911006115.pdf by guest on 24 February 2025 ERTR-7 and Desmin antibodies were purchased from Acris Antibodies.

Techniques: Ubiquitin Proteomics, Staining, Membrane

Journal: Translational Cancer Research

Article Title: Ovarian teratomas causing anti-N-methyl-D-aspartate receptor encephalitis: a case series from west China

doi: 10.21037/tcr-24-2126

Figure Lengend Snippet: Expression of NMDAR staining in the squamous epithelium and neural tissue of different groups. (A) NMDAR2B staining in the squamous epithelium (the arrow points to the squamous epithelium) was strongly positive in the encephalitis group. (B) NMDAR2A staining in the squamous epithelium (the arrow points to the squamous epithelium) was moderately positive in the encephalitis group. (C) NMDAR1 staining in the squamous epithelium (the arrow points to the squamous epithelium) was moderately positive in the encephalitis group. (D) NMDAR2B staining in the neural tissue was moderately positive in the only case of encephalitis group. (E) NMDAR2A staining in the neural tissue was moderately positive in the only case of encephalitis group. (F) NMDAR1 staining in the neural tissue was slightly positive in the only case of encephalitis group. (G) NMDAR2B staining in the neural tissue or squamous epithelium (as the arrow pointed) was slightly positive in a few cases of the IMT group. (H) NMDAR2A staining in the neural tissue or squamous epithelium (as the arrow pointed) was moderately positive in a few cases of the IMT group. (I) NMDAR1 staining in the neural tissue or squamous epithelium (as the arrow pointed) was slightly positive in a few cases of the IMT group. (J) NMDAR2B staining in the squamous epithelium (as the arrow pointed) was slightly positive in a few cases of the MT group. (K) NMDAR2A staining in the squamous epithelium (as the arrow pointed) was moderately positive in a few cases of the MT group. (L) NMDAR1 staining in the squamous epithelium (as the arrow pointed) was slightly positive in a few cases of the MT group. IMT, immature teratoma; MT, mature teratoma; NMDAR, N-methyl-D-aspartate receptor.

Article Snippet: The main antibodies (Abs) used were rabbit anti-human CD4 monoclonal antibody (dilution 1:100, Boster, China), rabbit anti-human CD20 polyclonal antibody (dilution 1:100, Boster, China), rabbit anti-human NMDAR2A antibody (dilution 1:100, Boster, China), rabbit anti-human NMDAR2B antibody (dilution 1:100, Tri-Eagle, China), rabbit anti-human NMDAR 1 antibody (dilution 1:100, Boster, China).

Techniques: Expressing, Staining